TargetScan is a target prediciton tool that predicts biological targets of miRNAs by searching for the presence of conserved 8mer and 7mer sites that match the seed region of each miRNA The target prediction software is frequently updated; PE, post eclosion PBM, post blood meal (D) Density plot of canonical miRNA seed matches in target mRNAs relative to the ligation site in all chimeras In the shuffled interactions (gray), each chimeric target region was randomly reassigned to a different miRNA (E) Predicted minimum free energy between miRNA and target mRNA found in chimeras The shuffled The nucleotides 28 in miRNA are termed 'seed,' which is the most crucial region for target recognition The seed region undergoes WatsonCrick base pairing with 3'UTR of mRNAs The degree of mRNA destabilization differs according to the class of the target site The different classes(of target sites to seed) are given below with increasing efficacy and preferential
Different Mirna Mrna Seed Site Interaction Patterns 6m Open I
